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Article

Enhanced discrimination of single nucleotide polymorphism in genotyping by phosphorothioate proofreading allele-specific amplification.

Analytical biochemistry - 1 Oct 2007

Hu Ya Jun, Li Zong Fang, Diamond Alan M

Abstract excerpt

There is a significant demand for sensitive, inexpensive, and flexible genotyping techniques that can be accomplished with reasonable throughput. Allele-specific amplification (ASA) has the advantage of combining the amplification and discrimination steps into a single reaction. However, mismatch amplification that occurs during traditional ASA limits its application for genotyping. Here, a modified ASA termed...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17631854
DOI: 10.1016/j.ab.2007.04.042

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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