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Article

Immunoglobulin gene conversion and hypermutation assay by FACs.

Sub-cellular biochemistry - 1 Jan 2006

Arakawa Hiroshi

Abstract excerpt

Immunoglobulin (Ig) gene conversion and hypermutation rates can be quantified by FACS. The spontaneous DT40 variant, C118, and C118-derived DT40 mutants can be used to analyze gene conversion phenotype. The C118 has a frameshift in its rearranged V segment. When this frameshift mutation is repaired by pseudogene-templated gene conversion, sIgM(-) DT40 reverts to sIgM (+) (Ig reversion assay). The sIgM (+)...

Topics

Animals
B-Lymphocytes
Cell Line
Cell Separation
Chickens
Flow Cytometry
Gene Conversion
Genes, Immunoglobulin
Mutation

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 17623918
DOI: 10.1007/978-1-4020-4896-8_23

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