Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Immunoglobulin gene conversion and hypermutation assay by FACs.

Sub-cellular biochemistry - 1 Jan 2006

Arakawa Hiroshi

Abstract excerpt

Immunoglobulin (Ig) gene conversion and hypermutation rates can be quantified by FACS. The spontaneous DT40 variant, C118, and C118-derived DT40 mutants can be used to analyze gene conversion phenotype. The C118 has a frameshift in its rearranged V segment. When this frameshift mutation is repaired by pseudogene-templated gene conversion, sIgM(-) DT40 reverts to sIgM (+) (Ig reversion assay). The sIgM (+)...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to B-Lymphocytes
Share to Cell Line
Share to Cell Separation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Chickens

Share to Flow Cytometry

Share to Gene Conversion

Share to Genes, Immunoglobulin

Share to Mutation

Source

Identifiers

PMID: 17623918
DOI: 10.1007/978-1-4020-4896-8_23

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Immunoglobulin gene conversion and hypermutation assay by FACs.PMID 17623918Read ↗

Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line.Semantic proximity Read ↗

Understanding the immunoglobulin locus specificity of hypermutation.Semantic proximity Read ↗

Uracil DNA Glycosylase Disruption Blocks Ig Gene Conversion and Induces Transition MutationsSemantic proximity Read ↗

Cellular and molecular studies of B cells exhibiting reverse somatic mutation throughout life.Semantic proximity Read ↗

Immunoglobulin gene conversion: insights from bursal B cells and the DT40 cell line.Semantic proximity Read ↗

Directed molecular evolution by somatic hypermutation.Semantic proximity Read ↗

Pseudogene-mediated gene conversion after CRISPR-Cas9 editing demonstrated by partial <i>CD33</i> conversion with <i>SIGLEC22P</i>Semantic proximity Read ↗

Somatic hypermutation of immunoglobulin ĸ light chain genesSemantic proximity Read ↗

Mechanistic properties of immunoglobulin somatic mutation.Semantic proximity Read ↗

Harmful somatic mutations: lessons from the dark side.Semantic proximity Read ↗

Immunoglobulin gene conversion or hypermutation: that's the question.Semantic proximity Read ↗

Protein evolution by hypermutation and selection in the B cell line DT40Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.