Pseudogene-mediated gene conversion after CRISPR-Cas9 editing demonstrated by partial <i>CD33</i> conversion with <i>SIGLEC22P</i>

Abstract excerpt

Although gene editing workflows typically consider the possibility of off-target editing, pseudogene-directed homology repair has not, to our knowledge, been reported previously. Here, we employed a CRISPR-Cas9 strategy for targeted excision of exon 2 in CD33 in U937 human monocyte cell line. Candidate clonal cell lines were screened by using a clinically relevant antibody known to label the IgV domain encoded by...

Topics

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• Cell Line, Tumor
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• Gene Editing
• Leukemia, Myeloid, Acute
• Polymorphism, Single Nucleotide
• Sialic Acid Binding Ig-like Lectin 3
• Viral Infectious Diseases and Gene Expression in Insects

Identifiers and source

Literature Corpus work

7c35e0f4-b436-55d8-b7e5-a74b7965f9a3

DOI

10.1101/2021.04.20.440641