Directed evolution of the nonribosomal peptide synthetase AdmK generates new andrimid derivatives in vivo.

Abstract excerpt

Many lead compounds in the search for new drugs derive from peptides and polyketides whose similar biosynthetic enzymes have been difficult to engineer for production of new derivatives. Problems with generating multiple analogs in a single experiment along with lack of high-throughput methods for structure-based screening have slowed progress in this area. Here, we use directed evolution and a multiplexed assay...

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