Article
A gain-of-function screen for genes that influence axon guidance identifies the NF-kappaB protein dorsal and reveals a requirement for the kinase Pelle in Drosophila photoreceptor axon targeting.
Genetics - 1 Aug 2007
Mindorff Elizabeth N, O'Keefe David D, Labbé Alain, Yang Jennie Ping, Ou Yimiao, Yoshikawa Shingo, van Meyel Donald J
Abstract excerpt
To identify novel regulators of nervous system development, we used the GAL4-UAS misexpression system in Drosophila to screen for genes that influence axon guidance in developing embryos. We mobilized the Gene Search (GS) P element and identified 42 lines with insertions in unique loci, including leak/roundabout2, which encodes an axon guidance receptor and confirms the utility of our screen. The genes we...
Topics
- Animals
- Axons
- Base Sequence
- DNA Primers
- Drosophila Proteins
- Drosophila melanogaster
- Female
- Genes, Insect
- Male
- Motor Neurons
- Mutation
- NF-kappa B
- Nuclear Proteins
- Phenotype