Selective amplification of rare mutations using locked nucleic acid oligonucleotides that competitively inhibit primer binding to wild-type DNA.

Abstract excerpt

Detection of mutated genomic DNA from cancer cells circulating in blood may improve tumor staging and patient selection for targeted therapy. However, the task of detecting a few mutated cells in the presence of a large excess of wild-type cells requires a sensitive and selective assay. We describe a novel approach to detect circulating melanoma cells harboring a common point mutation in the BRAF kinase. In the...

Topics

• Biomarkers, Tumor
• Cell Line
• DNA Primers
• DNA, Neoplasm
• Genetic Testing
• Humans
• Keratinocytes
• Melanoma
• Mutation
• Nucleic Acid Amplification Techniques
• Oligonucleotides