Article
High-sensitivity PCR method for detecting BRAF V600E mutations in metastatic colorectal cancer using LNA/DNA chimeras to block wild-type alleles.
Analytical and bioanalytical chemistry - 1 Apr 2014
Chen Dong, Huang Jun-Fu, Xia Han, Duan Guang-Jie, Chuai Zheng-Ran, Yang Zhao, Fu Wei-Ling, Huang Qing
Abstract excerpt
The response to epidermal growth factor receptor (EGFR)-targeted therapy in metastatic colorectal cancer (mCRC) is variable because of intra-tumor heterogeneity at the genetic level, and consequently, it is important to develop sensitive and selective assays to predict patient responses to therapy. Low-abundance BRAF V600E mutations are associated with poor response to treatment with EGFR inhibitors. We developed...
Topics
- Alleles
- Base Sequence
- Cell Line, Tumor
- Colorectal Neoplasms
- DNA
- Humans
- Molecular Sequence Data
- Mutation, Missense
- Nucleic Acids
- Polymerase Chain Reaction