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Continuous Hypermutation and Evolution of Luciferase Variants.

ACS chemical biology - 16 Jan 2026

Hadjian Tanya, Deshpande Ria A, Torrey Zachary R, Hammond Amelia W, Molina Rosana S, Ng Kevin, Liu Chang C, Prescher Jennifer A

Abstract excerpt

Several luciferases have been developed for imaging and biosensing, and the collection continues to grow as new applications are pursued. The current workflow for luciferase optimization, while successful, remains laborious and inefficient. Mutant libraries are generated in vitro and screened, "winning" mutants are picked by hand, and the isolated sequences are subjected to additional rounds of mutagenesis and...

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Directed Molecular Evolution
DNA Replication
Luciferases
Mutagenesis
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Engineering

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Identifiers

PMID: 41396131
DOI: 10.1021/acschembio.5c00621
PMCID: PMC13348083

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