Article
Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome.
Frontiers in bioscience : a journal and virtual library - 1 May 2007
Hewitt Chelsee Ann, Carmichael Catherine Louise, Wilkins Ella Jane, Cannon Ping Zhi Fang, Pritchard Melanie April, Scott Hamish Steele
Abstract excerpt
The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We...
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