Article
Rapid and reliable determination of transgene zygosity in mice by multiplex ligation-dependent probe amplification.
Transgenic research - 1 Dec 2009
Notini Amanda J, Li Ruili, Western Patrick S, Sinclair Andrew H, White Stefan J
Abstract excerpt
The ability to rapidly and unequivocally distinguish heterozygous from homozygous transgenic mice is an integral part of any breeding strategy. Here we describe a quick and simple protocol for determining the zygosity of transgenic mice at multiple loci in a single reaction. This involved the development of a multiplex ligation-dependent probe amplification (MLPA) probe mix to simultaneously measure common...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.