Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries.

Protein engineering, design & selection : PEDS - 1 May 2007

Herman Asael, Tawfik Dan S

Abstract excerpt

The directed evolution of proteins has benefited greatly from site-specific methods of diversification such as saturation mutagenesis. These techniques target diversity to a number of chosen positions that are usually non-contiguous in the protein's primary structure. However, the number of targe...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Aryldialkylphosphatase
Share to Directed Molecular Evolution
Share to DNA-Cytosine Methylases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Library

Share to Mutagenesis, Site-Directed

Share to Mutation

Share to Oligonucleotides

Share to Substrate Specificity

Source

Identifiers

PMID: 17483523
DOI: 10.1093/protein/gzm014

Genes

PON1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-17483523-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries.PMID 17483523Read ↗

DNA shuffling method for generating highly recombined genes and evolved enzymes.Semantic proximity Read ↗

Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.Semantic proximity Read ↗

Synthetic gene libraries: in search of the optimal diversity.Semantic proximity Read ↗

Exploiting models of molecular evolution to efficiently direct protein engineering.Semantic proximity Read ↗

Optimization of multi-site nicking mutagenesis for generation of large, user-defined combinatorial libraries.Semantic proximity Read ↗

Directed enzyme evolution via small and effective neutral drift libraries.Semantic proximity Read ↗

Directed evolution through DNA shuffling for the improvement and understanding of genes and promoters.Semantic proximity Read ↗

Correcting errors in synthetic DNA through consensus shuffling.Semantic proximity Read ↗

Massively parallel single-nucleotide mutagenesis using reversibly terminated inosine.Semantic proximity Read ↗

Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases.Semantic proximity Read ↗

Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis.Semantic proximity Read ↗

Directed evolution of sulfotransferases and paraoxonases by ancestral libraries.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.