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Article

Directed enzyme evolution via small and effective neutral drift libraries.

Nature methods - 1 Nov 2008

Gupta Rinkoo D, Tawfik Dan S

Abstract excerpt

Small libraries for directed evolution can be obtained by neutral drifts that maintain the protein's original function, yielding highly polymorphic, stable and evolvable variants. We describe methods for preparing such libraries, using serum paraoxonase (PON1). An optimized GFP variant fused to PON1 reported levels of soluble, functional enzyme, enabling selection by flow cytometry and identification of enzyme...

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PMID: 18931667
DOI: 10.1038/nmeth.1262

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PON1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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