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Article

Enhancing multiplex genome editing by natural transformation (MuGENT) via inactivation of ssDNA exonucleases.

Nucleic acids research - 7 Jul 2017

Dalia Triana N, Yoon Soo Hun, Galli Elisa, Barre Francois-Xavier, Waters Christopher M, Dalia Ankur B

Abstract excerpt

Recently, we described a method for multiplex genome editing by natural transformation (MuGENT). Mutant constructs for MuGENT require large arms of homology (>2000 bp) surrounding each genome edit, which necessitates laborious in vitro DNA splicing. In Vibrio cholerae, we uncover that this requirement is due to cytoplasmic ssDNA exonucleases, which inhibit natural transformation. In ssDNA exonuclease mutants, one...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28575400
DOI: 10.1093/nar/gkx496
PMCID: PMC5499599

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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