Article
Incidence of Harvey ras oncogene point mutations and their expression in methylbenzylnitrosamine-induced esophageal tumorigenesis.
Carcinogenesis - 1 Dec 1991
Barch D H, Jacoby R F, Brasitus T A, Radosevich J A, Carney W P, Iannaccone P M
Abstract excerpt
Activation of the ras oncogene was investigated in esophageal tumors induced by methylbenzylnitrosamine (MBN) in the Sprague-Dawley rat. DNA was extracted from grossly visible carcinogen-induced tumors. H-ras and K-ras gene sequences were then amplified by the polymerase chain reaction. Point mut...
Topics
- Adenine
- Animals
- Base Sequence
- Carcinogens
- Codon
- Dimethylnitrosamine
- Esophageal Neoplasms
- Esophagus
- Gene Expression Regulation, Neoplastic
- Genes, ras
- Guanine
- Immunohistochemistry
- Male
- Molecular Sequence Data
- Mutation
- Oncogene Protein p21(ras)
- Papilloma
- Rats