Article
Mutagenesis studies in transgenic Xenopus intermediate pituitary cells reveal structural elements necessary for correct prion protein biosynthesis.
Developmental neurobiology - 1 May 2007
van Rosmalen Jos W G, Martens Gerard J M
Abstract excerpt
The cellular prion protein (PrP(C)) is generally accepted to be involved in the development of prion diseases, but its physiological role is still under debate. To obtain more insight into PrP(C) functioning, we here used stable Xenopus transgenesis in combination with the proopiomelanocortin (POMC) gene promoter to express mutated forms of Xenopus PrP(C) fused to the C-terminus of the green fluorescent protein...
Topics
- Animals
- Animals, Genetically Modified
- Blotting, Western
- Glycosylation
- Glycosylphosphatidylinositols
- Green Fluorescent Proteins
- Immunoprecipitation
- Melanotrophs
- Microscopy, Electron, Transmission
- Mutagenesis
- Mutation
- Prions
- Pro-Opiomelanocortin
- Promoter Regions, Genetic
- Xenopus laevis