Article
Cellular phenotyping of secretory and nuclear prion proteins associated with inherited prion diseases.
The Journal of biological chemistry - 8 Mar 2002
Lorenz Holger, Windl Otto, Kretzschmar Hans A
Abstract excerpt
The pathogenic mechanisms leading from mutations in the prion protein (PrP) gene to infectious disease are not understood. To investigate the possibility that cellular processing of mutant prion protein may contribute to the formation of infectious particles, a mouse PrP model system has been established using the green fluorescent protein. Three novel PrP mutants were examined employing this model system and...
Topics
- Animals
- Blotting, Western
- Brefeldin A
- Cell Nucleus
- Creutzfeldt-Jakob Syndrome
- Detergents
- Green Fluorescent Proteins
- Humans
- Luminescent Proteins
- Mice
- Microscopy, Confocal
- Mutation
- Phenotype
- Point Mutation
- Prion Diseases
- Prions
- Protein Binding
- Recombinant Fusion Proteins