Article
Use of a novel Förster resonance energy transfer method to identify locations of site-bound metal ions in the U2-U6 snRNA complex.
Nucleic acids research - 1 Jan 2007
Yuan Faqing, Griffin Laura, Phelps LauraJane, Buschmann Volker, Weston Kenneth, Greenbaum Nancy L
Abstract excerpt
U2 and U6 snRNAs pair to form a phylogenetically conserved complex at the catalytic core of the spliceosome. Interactions with divalent metal ions, particularly Mg(II), at specific sites are essential for its folding and catalytic activity. We used a novel Förster resonance energy transfer (FRET) method between site-bound luminescent lanthanide ions and a covalently attached fluorescent dye, combined with...
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