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Article

A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo.

Nucleic acids research - 25 Jan 2018

Berry Katherine E, Hochschild Ann

Abstract excerpt

The interaction of RNA molecules with proteins is a critical aspect of gene regulation across all domains of life. Here, we report the development of a bacterial three-hybrid (B3H) assay to genetically detect RNA-protein interactions. The basis for this three-hybrid assay is a transcription-based bacterial two-hybrid assay that has been used widely to detect and dissect protein-protein interactions. In the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 29140461
DOI: 10.1093/nar/gkx1086
PMCID: PMC5778611

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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