Article
Modified peptide selection in vitro by introduction of a protein-RNA interaction.
Protein engineering - 1 Dec 2003
Sawata Shinya Y, Taira Kazunari
Abstract excerpt
The ribosome display system is a very effective and powerful tool for in vitro screening of transcribed mRNAs that encode proteins (or peptides) with specific (known or unknown) functions. The system depends on the stability of ribosome-mRNA complexes that have been formed as a result of the removal of a stop codon. To assess the general applicability of the system, we examined the stability of ribosome-mRNA...
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