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Article

Analyzing mRNA-protein complexes using a yeast three-hybrid system.

Methods (San Diego, Calif.) - 1 Feb 2002

Bernstein David S, Buter Natascha, Stumpf Craig, Wickens Marvin

Abstract excerpt

RNA-protein interactions are essential for the proper execution and regulation of every step in the life of a eukaryotic mRNA. Here we describe a three-hybrid system in which RNA-protein interactions can be analyzed using simple phenotypic or enzymatic assays in Saccharomyces cerevisiae. The system can be used to detect or confirm an RNA-protein interaction, to analyze RNA-protein interactions genetically, and to...

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PMID: 12054889
DOI: 10.1016/S1046-2023(02)00015-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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