Article
Dissociation of a native dimer to a molten globule monomer. Effects of pressure and dilution on the association equilibrium of arc repressor.
Journal of molecular biology - 20 Jan 1992
Silva J L, Silveira C F, Correia Júnior A, Pontes L
Abstract excerpt
The monomer-dimer association reaction of Arc repressor was studied by pressure-induced dissociation and by dilution. The dissociation was measured by the decrease (red shift) in the average energy of emission of the tryptophan fluorescence. Pressure dissociation also promoted a decrease in the excited-state lifetime of the single tryptophanyl residue, Trp14. These observations suggest that Trp14 becomes exposed...
Topics
- Anisotropy
- DNA-Binding Proteins
- Fluorescence
- Hydrostatic Pressure
- Macromolecular Substances
- Models, Chemical
- Mutation
- Protein Conformation
- Protein Denaturation
- Repressor Proteins