Article
Pressure effect on the structure of the Tet repressor protein TetR(B).
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 1 Nov 2002
Marian Dan-Thomas, Leypold Clemens, Kunz Michael, Schneider Siegfried, Schubert Peter, Scholz Oliver, Hillen Wolfgang
Abstract excerpt
The fluorescence of two single tryptophan (trp) mutants of the TetR(B)dimer protein was monitored for hydrostatic pressures 0.1 MPa < p < 300 MPa. In mutant W170, in which trp interacts with segments of both subunits, the fitted fluorescence lifetimes vary both with pressure and observation wavelength. In contrast, the lifetimes are fairly independent of both parameters in the case of W171, in which trp is...
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