Article
DNA sequence requirements for generating paused polymerase at the start of hsp70.
Genes & development - 1 Feb 1992
Lee H, Kraus K W, Wolfner M F, Lis J T
Abstract excerpt
RNA polymerase II is transcriptionally engaged but paused approximately 25 nucleotides from the start site of the hsp70 gene of Drosophila melanogaster in uninduced (non-heat-shocked) flies. Here, we identify regions of the hsp70 promoter that are required for formation of this paused polymerase. Various hsp70 promoter sequences are substituted for promoter sequences of a yolk protein gene, yp1, which, in males,...
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