Article
A novel class of mutations that affect DNA replication in E. coli.
Molecular microbiology - 1 Apr 2007
Nordman Jared, Skovgaard Ole, Wright Andrew
Abstract excerpt
Over-initiation of DNA replication in cells containing the cold-sensitive dnaA(cos) allele has been shown to lead to extensive DNA damage, potentially due to head-to-tail replication fork collisions that ultimately lead to replication fork collapse, growth stasis and/or cell death. Based on the assumption that suppressors of the cold-sensitive phenotype of the cos mutant should include mutations that affect the...
Topics
- Bacterial Proteins
- Cold Temperature
- DNA Replication
- DNA Transposable Elements
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli K12
- Escherichia coli Proteins
- Flow Cytometry
- Hydroxyurea
- Mutagenesis, Insertional