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Article

Isolation and characterization of novel cold-sensitive dnaA mutants of Escherichia coli.

FEMS microbiology letters - 15 Jul 1999

Guo L, Katayama T, Seyama Y, Sekimizu K, Miki T

Abstract excerpt

We developed an efficient method for isolation of novel dnaA mutations based on PCR mutagenesis in the presence of manganese ion and shuffling of dnaA-carrying plasmids in a dnaA deletion host bacterium. Using this system, we obtained 30 cold-sensitive mutants from 4000 clones carrying plasmids with a mutagenized dnaA gene. All 27 cold-sensitive mutants analyzed were defective in DNA replication; none had a...

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Identifiers

PMID: 10427718
DOI: 10.1111/j.1574-6968.1999.tb13684.x

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