Article
Unique nucleotide differences in the conserved 3' termini of brome mosaic virus RNAs are maintained through their optimization of genome replication.
Virology - 1 Mar 1992
Duggal R, Rao A L, Hall T C
Abstract excerpt
To explore the functionality and conservation of specific base differences in the 3' 200 nucleotides of brome mosaic virus (BMV) RNA-1 (1t) and RNA-2 (2t) with respect to the 3' end of RNA-3 (3t), all possible permutations were used to exchange these regions among the genomic RNAs. When all RNAs bore the 1t promoter, total RNA accumulation was only 15% of wild type; when the 2t or 3t promoter was present on all...
Topics
- Base Composition
- Base Sequence
- Blotting, Northern
- Genetic Variation
- Molecular Sequence Data
- Mosaic Viruses
- Mutagenesis, Insertional
- Nucleic Acid Conformation
- Plasmids
- Promoter Regions, Genetic
- Protoplasts