Article
Emergence of distinct brome mosaic virus recombinants is determined by the polarity of the inoculum RNA.
Journal of virology - 1 May 2012
Kwon Sun-Jung, Rao A L N
Abstract excerpt
Despite overwhelming interest in the impact exerted by recombination during evolution of RNA viruses, the relative contribution of the polarity of inoculum templates remains poorly understood. Here, by agroinfiltrating Nicotiana benthamiana leaves, we show that brome mosaic virus (BMV) replicase is competent to initiate positive-strand [(+)-strand] synthesis on an ectopically expressed RNA3 negative strand [(-)...
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