Dissecting the requirement for subgenomic promoter sequences by RNA recombination of brome mosaic virus in vivo: evidence for functional separation of transcription and recombination.

Abstract excerpt

Previously, we and others mapped an increased homologous recombination activity within the subgenomic promoter (sgp) region in brome mosaic virus (BMV) RNA3. In order to correlate sgp-mediated recombination and transcription, in the present work we used BMV RNA3 constructs that carried altered sgp repeats. We observed that the removal or extension of the poly(U) tract reduced or increased recombination,...

Topics

• Base Sequence
• Bromovirus
• Gene Expression Regulation, Viral
• Genome, Viral
• Molecular Sequence Data
• Mutation
• Promoter Regions, Genetic
• RNA, Viral
• Recombination, Genetic
• Transcription, Genetic