Article
A minimal RNA promoter for minus-strand RNA synthesis by the brome mosaic virus polymerase complex.
Journal of molecular biology - 26 Feb 1999
Chapman M R, Kao C C
Abstract excerpt
The approximately 150 nt tRNA-like structure present at the 3' end of each of the brome mosaic virus (BMV) genomic RNAs is sufficient to direct minus-strand RNA synthesis. RNAs containing mutations in the tRNA-like structure that decrease minus-strand synthesis were tested for their ability to interact with RdRp (RNA-dependent RNA polymerase) using a template competition assay. Mutations that are predicted to...
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