Article
Structure of yeast pGKL 128-kDa killer-toxin secretion signal sequence. Processing of the 128-kDa killer-toxin-secretion-signal-alpha-amylase fusion protein.
European journal of biochemistry - 1 Feb 1992
Tokunaga M, Kawamura A, Omori A, Hishinuma F
Abstract excerpt
The linear double-stranded DNA plasmid pGKL1 in yeast encodes a killer toxin consisting of 97-kDa, 31-kDa and 28-kDa subunits. A 128-kDa protein precursor of the 97-kDa and 31-kDa subunits, was first synthesized with a 29-amino-acid extension at its NH2-terminus as a secretion signal sequence. In...
Topics
- Amino Acid Sequence
- Blotting, Western
- DNA, Fungal
- Genes, Fungal
- Killer Factors, Yeast
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Mycotoxins
- Plasmids
- Precipitin Tests
- Protein Precursors
- Protein Processing, Post-Translational
- Protein Sorting Signals
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- alpha-Amylases