Article
Secretion of killer toxin encoded on the linear DNA plasmid pGKL1 from Saccharomyces cerevisiae.
The Journal of biological chemistry - 5 Oct 1990
Tokunaga M, Kawamura A, Kitada K, Hishinuma F
Abstract excerpt
By the kar1-mediated cytoduction, linear double-stranded DNA plasmids pGKL1 and pGKL2, encoding killer toxin complex, have been successfully transferred to the recipient strains with about 30% frequency. The killer toxin was found to be secreted through the normal yeast secretory pathway by intro...
Topics
- Animals
- Chromosome Deletion
- Dogs
- Endoplasmic Reticulum
- Fungal Proteins
- Killer Factors, Yeast
- Macromolecular Substances
- Molecular Weight
- Mutation
- Mycotoxins
- Pancreas
- Plasmids
- Protein Biosynthesis
- Protein Processing, Post-Translational
- Protein Sorting Signals
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins