Article
Efficient secretion in yeast based on fragments from K1 killer preprotoxin.
Yeast (Chichester, England) - 1 Apr 1992
Cartwright C P, Zhu Y S, Tipper D J
Abstract excerpt
The alpha and beta components of the secreted K1 killer toxin of Saccharomyces cerevisiae are derived from residues 45-147 and 234-316, respectively, of the 316 residue preprotoxin (ppTox). The beta N-terminus is produced by Kex2 cleavage after Lys Arg233; when beta la (the mature sequence of beta-lactamase) is fused at this site and the fusion is expressed from the PGK promoter in pDT17, a multicopy plasmid,...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA Mutational Analysis
- Golgi Apparatus
- Killer Factors, Yeast
- Molecular Sequence Data
- Mutation
- Mycotoxins
- Peptide Fragments
- Proprotein Convertases
- Recombinant Fusion Proteins