Article
Isolation and characterization of the K6 type yeast killer protein.
Microbios - 1 Jan 1999
Izgü F, Altinbay D, Sağiroğlu A K
Abstract excerpt
The optimum production of K6 type yeast killer protein by Kluyveromyces fragilis NCYC 587 occurred at pH 4.0-4.4 and at 22-24 degrees C in a killer-zone assay test. The K6 killer protein was concentrated by acetone precipitation of the culture supernatant and purified by native polyacrylamide rod gel electrophoresis. The protein migrated as a single band on discontinuous gradient SDS polyacrylamide gel...
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