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Article

Structural requirements of Bacillus subtilis alpha-amylase signal peptide for efficient processing: in vivo pulse-chase experiments with mutant signal peptides.

Journal of bacteriology - 1 Jul 1993

Sakakibara Y, Tsutsumi K, Nakamura K, Yamane K

Abstract excerpt

The Bacillus subtilis alpha-amylase signal peptide consists of 33 amino acids from its translation initiation site. To analyze the structural requirements for efficient processing of the signal peptide, single and repeated Ala-X-Ala sequences and their modifications were introduced into B. subtilis alpha-amylase signal peptides of different lengths and the mature thermostable alpha-amylase. Then the cleavage...

Read the complete abstract on PubMed

Topics

Alanine
Amino Acid Sequence
Amino Acids
Bacillus subtilis
Base Sequence
Enzyme Stability

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Escherichia coli

Kinetics

Molecular Sequence Data

Mutation

Protein Precursors

Protein Processing, Post-Translational

Protein Sorting Signals

Sequence Analysis

Sequence Homology, Amino Acid

Structure-Activity Relationship

Valine

alpha-Amylases

Source

Open in PubMed Open full text

Identifiers

PMID: 8320234
DOI: 10.1128/jb.175.13.4203-4212.1993
PMCID: PMC204850

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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