Mutation(s) of the thymidylate synthase gene of human adenocarcinoma cells causes a thymidylate synthase-negative phenotype that can be attenuated by exogenous folates.

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Article

Mutation(s) of the thymidylate synthase gene of human adenocarcinoma cells causes a thymidylate synthase-negative phenotype that can be attenuated by exogenous folates.

Cancer research - 1 Feb 1992

Houghton P J, Rahman A, Will C L, Dolnick B J, Houghton J A

Abstract excerpt

Biological characterization of a human colon adenocarcinoma cell line deficient in thymidylate synthase (TS-) is described. The clone, designated TS-C1/C1, was derived from the parental line GC3/C1 by selection in medium containing aminopterin, thymidine (dThd), and low concentrations of 5-formyltetrahydrofolate (5-CHO-H4PteGlu), and was subsequently reselected by single-step cloning in 500 microM methotrexate in...

Topics

Adenocarcinoma
Animals
Base Sequence
Cell Division
Cell Line
Colonic Neoplasms
Folic Acid
Humans
Kinetics
Leucovorin
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mice, Nude

Mitotic Index

Molecular Sequence Data

Mutation

Neoplasm Transplantation

Oligodeoxyribonucleotides

Phenotype

Source

Open in PubMed

Identifiers

PMID: 1732043

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1732043-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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