Article
Functional effects of a naturally occurring amino acid substitution in human thymidylate synthase.
Molecular pharmacology - 1 Aug 1993
Hughey C T, Barbour K W, Berger F G, Berger S H
Abstract excerpt
A major mechanism underlying the cytotoxicity of fluoropyrimidine analogs such as 5-fluorouracil and 5-fluoro-2'-deoxyuridine (FdUrd) occurs via the formation of 5-fluoro-2'-deoxyuridylate (FdUMP), a tight-binding inhibitor of thymidylate synthase (TS). Genetic variation in the structure of the T...
Topics
- Base Sequence
- Binding Sites
- Floxuridine
- Fluorouracil
- Histidine
- Humans
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Temperature
- Tetrahydrofolates
- Thymidylate Synthase
- Tumor Cells, Cultured
- Tyrosine