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Article

Random sequence mutagenesis and resistance to 5-fluorouridine in human thymidylate synthases.

The Journal of biological chemistry - 2 Oct 1998

Landis D M, Loeb L A

Abstract excerpt

Thymidylate synthase (TS) catalyzes the methylation of dUMP to dTMP and is the target for the widely used chemotherapeutic agent 5-fluorouracil. We used random sequence mutagenesis to replace 13 codons within the active site of TS and obtain variants that are resistant to 5-fluorodeoxyuridine (5-...

Topics

Amino Acid Sequence
Binding Sites
Cell Survival
Deoxyuracil Nucleotides
Drug Resistance
Enzyme Inhibitors
Escherichia coli
Humans
Kinetics
Molecular Sequence Data
Mutagenesis
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Binding

Sequence Analysis, DNA

Tetrahydrofolates

Thymidylate Synthase

Uridine

Source

Identifiers

PMID: 9748254
DOI: 10.1074/jbc.273.40.25809

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9748254-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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