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Article

Random sequence mutagenesis and resistance to 5-fluorouridine in human thymidylate synthases.

The Journal of biological chemistry - 2 Oct 1998

Landis D M, Loeb L A

Abstract excerpt

Thymidylate synthase (TS) catalyzes the methylation of dUMP to dTMP and is the target for the widely used chemotherapeutic agent 5-fluorouracil. We used random sequence mutagenesis to replace 13 codons within the active site of TS and obtain variants that are resistant to 5-fluorodeoxyuridine (5-FdUR). The resulting random library was selected for its ability to complement a TS-deficient Escherichia coli strain,...

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PMID: 9748254
DOI: 10.1074/jbc.273.40.25809

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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