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Article

Human immunodeficiency virus type 1 envelope gene structure and diversity in vivo and after cocultivation in vitro.

Journal of virology - 1 Feb 1992

Kusumi K, Conway B, Cunningham S, Berson A, Evans C, Iversen A K, Colvin D, Gallo M V, Coutre S, Shpaer E G

Abstract excerpt

Nested-primer polymerase chain reaction (PCR) has been applied to the molecular cloning of 4.6-kb half-genome fragments of human immunodeficiency virus type 1 (HIV-1) taken directly from the peripheral blood mononuclear cells (PBMC) of an individual with neurological symptoms of HIV-1 infection. In a similar manner, gp120-coding portions of the envelope gene were cloned after PBMC from the same blood sample were...

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Identifiers

PMID: 1731112
DOI: 10.1128/JVI.66.2.875-885.1992
PMCID: PMC240788

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