Article
Human immunodeficiency virus type 1 envelope gene structure and diversity in vivo and after cocultivation in vitro.
Journal of virology - 1 Feb 1992
Kusumi K, Conway B, Cunningham S, Berson A, Evans C, Iversen A K, Colvin D, Gallo M V, Coutre S, Shpaer E G
Abstract excerpt
Nested-primer polymerase chain reaction (PCR) has been applied to the molecular cloning of 4.6-kb half-genome fragments of human immunodeficiency virus type 1 (HIV-1) taken directly from the peripheral blood mononuclear cells (PBMC) of an individual with neurological symptoms of HIV-1 infection....
Topics
- Acquired Immunodeficiency Syndrome
- Adult
- Amino Acid Sequence
- Base Sequence
- Cells, Cultured
- Cloning, Molecular
- DNA, Viral
- Genes, Viral
- Genetic Variation
- Genome, Viral
- HIV-1
- Humans
- Male
- Molecular Sequence Data
- Oligodeoxyribonucleotides
