Molecular characterization of human immunodeficiency virus type 1 cloned directly from uncultured human brain tissue: identification of replication-competent and -defective viral genomes.

Abstract excerpt

All presently available replication-competent proviral clones of human immunodeficiency virus type 1 (HIV-1) are derived from cell culture-amplified virus. Since tissue culture is highly selective for viral strains with an in vitro growth advantage, such clones may not be representative of the biologically relevant virus present in vivo. In this study, we report the molecular cloning and genotypic...

Topics

• AIDS Dementia Complex
• Amino Acid Sequence
• Bacteriophage lambda
• Base Sequence
• Blotting, Southern
• Blotting, Western
• Brain
• Cells, Cultured
• Cloning, Molecular
• DNA, Circular