Article
Genetic comparison of human immunodeficiency virus (HIV-1) isolates by polymerase chain reaction.
Journal of acquired immune deficiency syndromes - 1 Jan 1991
McCutchan F E, Sanders-Buell E, Oster C W, Redfield R R, Hira S K, Perine P L, Ungar B L, Burke D S
Abstract excerpt
Amplification of DNA by polymerase chain reaction (PCR) is influenced by the homology of oligonucleotide primers with the DNA template. We have developed a procedure, termed anchored PCR, whereby nucleotide sequence alterations in the template can be directly related to the quantity of amplified product. Genetic variation in the human immunodeficiency virus HIV-1 has been studied using anchored PCR. In four field...
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