Expression and simple, one-step purification of fragile histidine triad (Fhit) tumor suppressor mutant forms in Escherichia coli and their interaction with protoporphyrin IX.

Abstract excerpt

The product of human fragile histidine triad (FHIT) gene is a tumor suppressor protein of still largely unknown cellular background. We have shown previously that it binds protoporphyrin IX (a photosensitizer) which alters its enzymatic activity in vitro. Fhit, diadenosine triphosphate (Ap3A) hydrolase, possesses the active site with histidine triad His-phi-His-phi-His-phiphi. So-called histidine Fhit mutants...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Acid Anhydride Hydrolases
• Share to Blotting, Western