Article
Orthogonal protein purification--expanding the repertoire of GST fusion systems.
Protein expression and purification - 1 Jan 2008
Viljanen Johan, Larsson Jenny, Broo Kerstin S
Abstract excerpt
We have previously developed a labeling scheme that can be used to site-specifically link human glutathione transferases (hGSTs) from the alpha class to chemical entities such as fluorophores and aldehydes. The reagents are in-house synthesized derivatives of glutathione (GS-derivatives). We have focused on a lysine mutant of hGST A1:A216K. In this study, we wanted to utilize these findings and improve on protein...
Topics
- Acylation
- Aldehydes
- Base Sequence
- Binding Sites
- Catalysis
- Chromatography, Affinity
- Codon
- Coumarins
- Crystallization
- Dimerization
- Enzyme Stability
- Escherichia coli