Article
In vitro trans-translation of Thermus thermophilus: ribosomal protein S1 is not required for the early stage of trans-translation.
RNA (New York, N.Y.) - 1 Apr 2007
Takada Kazuma, Takemoto Chie, Kawazoe Masahito, Konno Takayuki, Hanawa-Suetsugu Kyoko, Lee Sungga, Shirouzu Mikako, Yokoyama Shigeyuki, Muto Akira, Himeno Hyouta
Abstract excerpt
Transfer-messenger RNA (tmRNA) plays a dual role as a tRNA and an mRNA in trans-translation, during which the ribosome replaces mRNA with tmRNA encoding the tag-peptide. These processes have been suggested to involve several tmRNA-binding proteins, including SmpB and ribosomal protein S1. To investigate the molecular mechanism of trans-translation, we developed in vitro systems using purified ribosome, elongation...
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