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Article

An inducible system for expression and validation of the specificity of short hairpin RNA in mammalian cells.

Nucleic acids research - 1 Jan 2007

Ma Hoi Tang, On Kin Fan, Tsang Yiu Huen, Poon Randy Y C

Abstract excerpt

RNA interference (RNAi) by means of short hairpin RNA (shRNA) has developed into a powerful tool for loss-of-function analysis in mammalian cells. The principal problem in RNAi experiments is off-target effects, and the most vigorous demonstration of the specificity of shRNA is the rescue of the RNAi effects with a shRNA-resistant target gene. This presents its own problems, including the unpredictable relative...

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Identifiers

PMID: 17234679
DOI: 10.1093/nar/gkl1109
PMCID: PMC1851631

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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