Article
Rapid conditional knock-down-knock-in system for mammalian cells.
Nucleic acids research - 1 Jan 2007
Hölzel Michael, Rohrmoser Michaela, Orban Mathias, Hömig Cornelia, Harasim Thomas, Malamoussi Anastassia, Gruber-Eber Anita, Heissmeyer Vigo, Bornkamm Georg, Eick Dirk
Abstract excerpt
RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly of multimolecular complexes. Replacing the endogenous protein with ectopic wild-type or mutant forms can exclude...
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