Article
Validation of short interfering RNA knockdowns by quantitative real-time PCR.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Tuzmen Sukru, Kiefer Jeff, Mousses Spyro
Abstract excerpt
RNA interference (RNAi) is a natural mechanism, that is triggered by the introduction of double-stranded RNA into a cell. The long double-stranded RNA is then processed into short interfering RNA (siRNA) that mediates sequence-specific degradation of homologous transcripts. This phenomenon can be exploited to experimentally trigger RNAi and downregulate gene expression by transfecting mammalian cells with...
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