Article
A system for stable expression of short interfering RNAs in mammalian cells.
Science (New York, N.Y.) - 19 Apr 2002
Brummelkamp Thijn R, Bernards René, Agami Reuven
Abstract excerpt
Mammalian genetic approaches to study gene function have been hampered by the lack of tools to generate stable loss-of-function phenotypes efficiently. We report here a new vector system, named pSUPER, which directs the synthesis of small interfering RNAs (siRNAs) in mammalian cells. We show that siRNA expression mediated by this vector causes efficient and specific down-regulation of gene expression, resulting...
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