Article
Separation of near full-length hepatitis C virus quasispecies variants from a complex population.
Journal of virological methods - 1 May 2007
Zhou Donghui, Fan Xiaofeng, Tan De, Xu Yanjuan, Tavis John E, Di Bisceglie Adrian M
Abstract excerpt
A long RT-PCR (LRP) protocol was developed recently for robust amplification of a near full-length HCV genomic sequence from clinical samples, followed by efficient cloning [Fan, X., Xu, Y., Di Biceglie, A.M., 2006. Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples. Biochem. Biophys. Res. Commun. 346, 1163-1172]. In the present study, the LRP protocol has been...
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