Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples.

Virology journal - 12 Feb 2013

Zhang Eileen Z, Bartels Doug J, Frantz J Dan, Seepersaud Sheila, Lippke Judith A, Shames Benjamin, Zhou Yi, Lin Chao, Kwong Ann, Kieffer Tara L

Abstract excerpt

BACKGROUND: Direct-acting antiviral (DAAs) agents for hepatitis C virus (HCV) span a variety of targets, including proteins encoded by the NS3/4A, NS4B, NS5A, and NS5B genes. Treatment with DAAs has been shown to select variants with sequence changes in the HCV genome encoding amino acids that may confer resistance to the treatment. In order to assess these effects in patients, a Reverse Transcription Polymerase...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antiviral Agents
Share to Drug Resistance, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genome, Viral

Share to Hepacivirus

Share to Hepatitis C

Share to Molecular Biology

Share to Plasma

Share to Virology

Source

Open in PubMed Open full text

Identifiers

PMID: 23402332
DOI: 10.1186/1743-422X-10-53
PMCID: PMC3575352

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples.PMID 23402332Read ↗

A near full-length open reading frame next generation sequencing assay for genotyping and identification of resistance-associated variants in hepatitis C virus.Semantic proximity Read ↗

A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame.Semantic proximity Read ↗

A genotype independent, full-genome reverse-transcription protocol for HCV genotyping and resistance testing.Semantic proximity Read ↗

A complete molecular biology assay for hepatitis C virus detection, quantification and genotyping.Semantic proximity Read ↗

Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b.Semantic proximity Read ↗

Development and Validation of a Template-Independent Next-Generation Sequencing Assay for Detecting Low-Level Resistance-Associated Variants of Hepatitis C Virus.Semantic proximity Read ↗

Genotype- and Subtype-Independent Full-Genome Sequencing Assay for Hepatitis C VirusSemantic proximity Read ↗

Virology analysis in HCV genotype 1-infected patients treated with the combination of simeprevir and TMC647055/ritonavir, with and without ribavirin, and JNJ-56914845.Semantic proximity Read ↗

Virologic Tools for HCV Drug Resistance TestingSemantic proximity Read ↗

A method for near full-length amplification and sequencing for six hepatitis C virus genotypes.Semantic proximity Read ↗

Hepatitis C virus RNA assays: current and emerging technologies and their clinical applications.Semantic proximity Read ↗

Identification of HCV Resistant Variants against Direct Acting Antivirals in Plasma and Liver of Treatment Naïve Patients.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.