A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame.

doi:10.1186/1743-422X-2-88

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Article

A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame.

Virology journal - 1 Dec 2005

Yao Ermei, Tavis John E

Abstract excerpt

BACKGROUND: Hepatitis C virus (HCV) is a pathogenic hepatic flavivirus with a single stranded RNA genome. It has a high genetic variability and is classified into six major genotypes. Genotype 1a and 1b cause the majority of infections in the USA. Viral genomic sequence information is needed to correlate viral variation with pathology or response to therapy. However, reverse transcription-polymerase chain...

Topics

DNA, Complementary
DNA, Viral
Genotype
Hepacivirus
Open Reading Frames
RNA, Viral
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Sequence Analysis

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Identifiers

PMID: 16321149
DOI: 10.1186/1743-422X-2-88
PMCID: PMC1325262

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